An Efficient Gene-Trap Method Using Poly A Trap Vectors and Characterization of Gene-Trap Events1

Trap characterization in composite of solid-liquid using dual-level trap model and TSDC method

Patterns of gene action in plant development revealed by enhancer trap and gene trap transposable elements.

The crucifer Arabidopsis thaliana has been used widely as a model organism for the study of plant development. We describe here the development of an efficient insertional mutagenesis system in Arabidopsis that permits identification of genes by their patterns of expression during development. Transposable elements of the Ac/Ds system carrying the GUS reporter gene have been designed to act as ...

Location as a Poverty Trap

In this paper, we ask “Can proximity to towns reduces poverty in rural areas?” For this study we have mapped all the villages in Orissa. The study is based on small area poverty estimation methodology as well as the secondary data covering 47,395 villages and 109 towns. Our main findings are (i) there is a relationship between the distance to nearest town and rural poverty and (ii) poverty leve...

A gene trap approach in Xenopus

The frog transgenesis technique ultimately promises to make mutagenesis possible through random insertion of plasmid DNA into the genome. This study was undertaken to evaluate whether a gene trap approach combined with transgenesis would be appropriate for performing insertional mutagenesis in Xenopus embryos. Firstly, we confirmed that the transgenic technique results in stable integration int...

Efficient gene tagging in Arabidopsis thaliana using a gene trap approach.

Large quantities of DNA sequence information about plant genes are rapidly accumulating in public databases, but to progress from DNA sequence to biological function a mutant allele for each of the genes ideally should be available. Here we describe a gene trap construct that allowed us to disrupt transcribed genes with a high efficiency in Arabidopsis thaliana. In the T-DNA vector used, the ex...